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Some blocks were embedded in plastic and semi-thin sections were collected for examination at higher resolution.
For Nissl staining following by stereological neuron counting, 10 µm thick paraffin sections were collected; for immunostaining, Fluoro-Jade B, and TUNEL analysis, 20 µm thick sections were collected on a cryostat.
Liver sections were collected for toxicogenomics analysis from all animals.
Two times five 10-μm sections were collected for DNA extraction.
Liver tissue sections were collected for histological examination or were flash frozen.
The intervening muscle sections were collected for subsequent RT PCR analysis.
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An identical set of fresh tissue sections was collected for routine bacterial culture and viral analysis.
Approximately 20 images from 5-6 normammarymary sections (2-3 sections from each normal patient) and 6-8 images for each BH, DCIS or IBC section were collected for a total of ~4500 images.
Every fourth section was collected for free-floating IHC.
Every 10th section was collected for sampling.
Every 20th section was collected for further histological analysis.
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