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The sections were assessed using light microscopy in a blinded manner by at least two of the authors.
The sections were assessed using a light microscope in a blinded fashion by at least two of the authors.
Comparisons of nonparametric data from the modified Mankin histological scoring of the stained tissue sections were assessed using the Mann–Whitney U-test.
Haematoxylin and eosin-stained sections were assessed using the modified Sydney system for evaluating gastric changes related to Helicobacter pylori [ 42].
The sections were assessed using a Nikon Eclipse 80i microscope at ×200 view and a Nikon Digital Sight DS-U1 camera (Nikon UK, Kingston on Thames, UK).
Fluorochrome labelled bone sections were assessed using a triple filter (Zeiss filter set no. 25, Zeiss Axioplan, Carl Zeiss AG) and a fluorescent lamp.
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Analysis of sections was assessed using a semi-quantitative method which differed depending on the antibody used.
The degree of homogeneity of the child (CIS) and family (FIS) sections was assessed using Cronbach's Alpha Coefficient.
Next, staining in whole tissue sections was assessed using the Allred scoring system, which consists of a combined score for intensity level (0 3) and proportion of positive cells (0 5).
The reliability of the UI symptom severity section and attitude section were assessed using Cronbach's alpha and was found to be 0.73 and 0.82, respectively.
Each section is assessed using multiple choice questions with 4 or 5 optional responses, of which only one is correct.
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