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ARC and FOS staining in fixed tissue sections were analyzed in GraphPad Prism 7 using a one-way ANOVA and compared to HC with Dunnett's multiple comparisons test.
Since, ANIT has been used as a cholestatic agent and it has been pointed out that an impairment of mitochondrial function is a cause of hepatocyte dysfunction leading to cholestatic liver injury, serum markers of liver injury were measured and liver sections were analyzed in ANIT-treated rats (i.p. 80 mg/kg/weeks16 weeks).
Sections were analyzed in blind by a specialized patho-nephrologist.
Sections were analyzed in a Leica DMLA microscope (Leica Microsystems, Bannockburn, IL) with fluorescent light.
Sections were analyzed in a Jeol 100 CX II electron microscope.
Thirty-seven species of the Paphiopedilum genus covering all seven sections were analyzed in this study.
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Surface morphology and microstrucure of deposited TiN in a cross section were analyzed in a field emission SEM (FE-SEM; Hitachi S-4300, Hitachi, Ltd, Chiyoda-ku, Japan) and TEM Philipss Tecnai 20, Philips, Amsterdam, The Netherlands).
For each case a total of three sections (10 images per section) were analyzed in order to estimate the average number of immunolabeled cells per unit area (mm2) and the average intensity of the immunostaining (corrected optical density).
At least 50 glomeruli per section were analyzed in a blinded manner.
For each mouse, a total of three sections (10 images per section) were analyzed in order to estimate the average number of immunolabeled cells per unit area (mm) or the average intensity of the immunoreactive signal (corrected pixel intensity).
The samples, prepared as described in " Quantification of the adsorption of probe compounds by SPME fiber" section, were analyzed in triplicate after 0, 0.5, 1, 2, 3, and 12 h of incubation at room temperature and the amount adsorbed at each incubation time was calculated.
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