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Following the treatments, the mammary glands were analysed by whole mount analysis and sections were analysed for the percentage of budding epithelial structures, proliferation and macrophage recruitment.
The sections were analysed for the extent of positive staining in remote, borderzone and infarct by light microscopy at ×400 magnification.
The sections were analysed for the presence or absence of aspirated material.
One to three sections were analysed for 4 animals per group (rapamycin vs vehicle).
Two sections were analysed for each case and from each composite image two fields were chosen for counting.
All sections were analysed for both minimal Feret's diameter and internally nucleated fibres.
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The sections were analysed from tumours obtained from each mouse and a representative slide is shown in the results section.
For each sample, about 100 sections were analysed, and for each section, the number of cellular sites of CPT accumulation was counted.
Adjacent sets of unfixed rat brain sections were analysed in triplicate for quantitative autoradiography for TSPO with 125I]-CLINDE, OX-42 (microglial activation) immunohistochemistry and cresyl violet (cell loss).
For each patient, three full-specimen sections were analysed, one slide each for TS, TP, and ERCC1.
The same H-&-E-stained sections were analysed as described above for quantification of erosions.
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