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A 'by-eye' assessment of all sections was conducted by an experienced toxicological pathologist.
To investigate the possibility of the ex vivo assessment of the molecular status of the tumour by employing the 11A4-680 and B9-800 combination, dual-spectral imaging of tumour sections was conducted and compared to conventional haematoxylin and eosin (H&E) staining.
Confocal imaging of fluorescent stained sections was conducted using a Zeiss LSM 510 Meta confocal system and Zeiss LSM 510 software (Version 4.0 SP2) with multi-track scanning.
To determine what cell type was responsible for increased γcyto-actin and βcyto-actin expression in mdx and ms-DKO skeletal muscle extracts, immunofluorescence analysis of quadriceps cross sections was conducted.
Osteoclast analysis in bone sections was conducted using Osteomeasure software (Osteometrics Inc., Atlanta GA).
Histopathological examination of tissue sections was conducted at the Experimental Animal Research Center, China Agricultural University.
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Twenty-five four-point bending tests on circular, rectangular and square hollow structural sections were conducted.
A series of tests on cold formed stainless steel square and rectangular hollow sections is conducted under end loading and interior loading using the same test setup as Zhao and Hancock and Young and Hancock.
Three pullout experiments for embedded IPE140 sections were conducted for this paper.
Only 35-40% of caesarean sections were conducted due to strong indications and for a majority of sections the indications were non-absolute.
This could indicate that the referral system is functional, such that complicated deliveries and caesarean sections are conducted in areas where available qualified personnel are located.
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