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In the preclinical context, assessment of near-infrared probes' distribution in tumors and organs has been made either with intact organs ex vivo or through imaging of tissue sections, using the same systems as used for noninvasive imaging of mice [9, 11].
Immunocytochemical labeling of cell cultures on coverslips was essentially similar to that described for tissue sections, using the same antibodies, but with primary and secondary antibody incubation times reduced to 2 4 hours, and Triton-X concentration in buffers reduced to 0.02%.
Next, we immunostained human eye sections using the same antibody.
A modified version for paraffin sections, using the same antibodies, is described in the subsequent section.
To confirm this point, we performed immunofluorescence staining of frozen sections using the same anti-URAT1 antibody.
We also confirmed this frequent association of HOT1 with telomeres in mouse testes sections using the same IF set-up.
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This analysis deals with East Cambridgeshire, the only centre to resurvey a repeated cross-section using the same methodology after five years.
Each section uses the same notation for these submatrices, but with different dimensions.
Section by section, use the same method to thin out your hair.
All cells were visualized in the same sections using the nuclear stain Hoechst 33258 (Invitrogen, H3569).
In order to quantify the total number of granular neurons in the SGZ-GCL, a Nissl staining was performed on brain sections prepared using the same fixation and cutting protocol described for immunohistochemical procedures.
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