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We would like to acknowledge the Histology Core at MMCRI for providing us with the tissue sections used in this study.
Human fetal brain sections used in this study were obtained from a midgestation fetus (21 weeks post gestation).
For all sections used in this study, the antigen retrieval procedure was performed by immersing the slides in citrate buffer (pH 6.0) and then heating the slides in a microwave oven for 10 min.
To examine this, we employed an immunohistochemical method originally developed by Makitie et al., providing sensitive and specific staining with very little background autofluorescence (Makitie et al. 2010), which is very effective with the formalin-fixed sections used in this study (Fragoso et al. 2012).
To prevent variable results yield by different batches of materials, tissue sections used in this study were stored in cryoprotectant (0.05 M phosphate buffer, 30%% ethylene glycol, 20%% glycerol; at −20 °C) such that sections of different sections of different age groups were processed at the same time.
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Pores were separated into residual primary intergranular pores, intragranular dissolution pores, and enlarged intergranular dissolution pores during the point counting of each thin section used in this study.
The cross-section used in this study was from an 80- to 90-year-old tree.
In addition, the direct strength method, which was developed for cold-formed steel members for certain cross-sections but not cover oval hollow sections, was used in this study.
Eighty orthodontic NiTi arch wires with round and rectangular cross sections were used in this study (Ortho Technology, Tampa, FL, USA).
Although both sections were completed, only the second section was used in this study.
The base-case simulation model in "Scenario 1: depletion to low pressure" section was used in this study.
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