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We then applied the Abercrombie formula (Abercrombie 1946), which uses the average measured diameter of the germ cell nuclei and the thickness of sections to correct for any double counting due to single cells appearing in two successive sections.
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They've all rushed off to the comment section to correct me for saying "assistant" captain instead of "alternate".
Telescope mirrors are commonly made parabolic or hyperbolic in section to correct the aberrations of the image.
To ensure unbiased estimate of objects counted, the method uses a guard zone at the bottom and top of each section to correct for objects plucked during sectioning, so the disector thickness is always smaller than the thickness of the section.
We have added a short paragraph to the introduction section to correct this.
The number of LC3A‐positive SLS was counted in the entire section at a magnification of 400×, and then, the number in each section was divided by the total number of fields included in the entire section to correct for different section sizes (Sivridis et al., 2013).
We then used the Abercrombie formula (Abercrombie 1946), which uses the average measured diameter of the germ cell nuclei (D) and the thickness of sections (S) to correct for any double counting due to single cells appearing in two successive sections, to obtain the true count (TC): TC = CC × S/(S+ D).
The remaining sections were mapped in relation to the center by serially applying the intervening adjacent section-to-section transforms and warped to correct for distortions resulting from the sectioning process or section folds.
Clearly, the fidelity of the nuclear cross-sections is essential to correct specification of shield design and sensitivity to cross-section error is important in guiding experimental validation of cross-section models and database.
Temperature-dependent CO2 absorption cross-sections are used to correct for laser and signal attenuation.
All counts were expressed as cells per mm or mm adjusted by Abercrombie's correction factor to correct for section thickness and mean cell size [ 28].
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