Sentence examples for sections to be processed from inspiring English sources
A standard diaminobenzidine protocol (0.05% diaminobenzidine and 0.003% H2O2) was used to develop the monkey sections to be processed for ultrastructural analysis.
Free-floating sections to be processed for single or double IHC labeling were first rinsed for 30 min in 0.02 M potassium PBS (KPBS; 16 mM K2HPO4, 3.5 mM KH2PO4, 150 mM NaCl, pH 7.4), incubated in 1% hydrogen peroxide for 30 min to block endogenous peroxidase and rinsed in KPBS.
Consequently, large numbers of sections from different animals have to be processed, raising a major technical problem, namely the loss of three-dimensional (3D) spatial consistency across brains.
A further series (also one-in-four sections) was collected in PBS to be processed for either serotonin or acetylcholinesterase staining.
Sections to be directly compared were processed together on the same slide.
In all cases, vibratome sections adjacent to the IHC sections were processed for in situ hybridisation (ISH) experiments designed to detect Cux2, Rorβ, Er81, Nurr1 and CTGF mRNA expression.
Sections were processed to paraffin by routine processing, 5 micron sections were stained with Haematoxylin and Eosin for assessment.
Five-micron sections were processed to perform the silver staining to visualize the nucleolar organizer regions and the argyrophilic proteins according to the guidelines of the "International committee on AgNOR quantitation" [ 22].
One series of sections was Nissl stained with cresyl violet, whereas a second series of sections was processed to visualize the marker SMI-32, as previously described (Wannier et al. 2005; Beaud et al. 2008).
Slides containing these sections were processed to reveal Nissl substance beginning with a brief fixation in 4% paraformaldehyde in 0.1M PB for 15 minutes after which they were washed in distilled water three times for 2 minutes per wash.
In experiments with tracer injections, one series of sections was processed to visualize boutons and labeled neurons, using standard bright-field or fluorescence immunohistochemical protocols, as described previously [27].
