Immunostaining of Pis1 in the tissue sections retrieved with citrate buffer at both low and high temperatures was faint, while the sections retrieved with Tris-EDTA buffer gave stronger Pis1 immunoreactivity and a desirable light background at high temperature.
After water washing, sections retrieved the antigen with citrate buffer pH 6.0 in microwave for thirteen minutes.
CD68 (KP1, DAKO, Denmark), CD14 (M0825 clone TUK4, DAKO), IL-1β (Abcam ab8320), IL-6 (Abcam ab9324) and TNF (PeproTech 500-M26) staining was performed on sections retrieved in PT101 Link Pre-Treatment Module (Dako), as previously described [ 37].
More in depth, Bamboo and Georoy, the two protocols considered that will be described in the following sections, retrieve resources according to a distributed mechanism where, to speed up the lookup process and make it suitable to an unreliable scenario like the one addressed by our study, a replication methodology for managing multiple copies of the same resource has been introduced.
(2) The MS spectra were downloaded from Metabolight [44] and the OpenMS workflow described in the "Methods" section retrieved a total of 800 distinct peaks.
This classification was performed according to predictions in KEGG for the distribution of reactions in the collection of species (as described earlier in the section 'Retrieving a set of the human metabolic reactions').
My only serious comment would be that the authors refer to species' taxonomy as if it perfectly reflects species' phylogeny (e.g. the end of the first paragraph in the introduction and the first paragraph in the section "retrieve higher taxonomic names").
After 2 weeks, the experimental sections were retrieved, sliced into thin sections 3.5 μm in average thickness, decalcified, and stained with hematoxylin eosin.
Tissue sections were retrieved using Bond Epitope Retrieval Solution 1 (Vision BioSystems) and stained with antibodies against hnRNP K (mouse monoclonal antibody, 1 300 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA) and MMP-12 (goat polyclonal antibody, 1 10 dilution; Santa Cruz Biotechnology).
For staining with an anti-human CD163 or CD204 antibody, deparaffinized sections were retrieved by incubation with target retrieval solution (Dako, Carpinteria, CA, USA) or citrate buffer pH 6 for 5 minutes with a microwave oven, respectively.
The antigens in the sections were retrieved using a decloaking chamber (Biocare Medical, Walnut Creek, CA, USA) with a retrieval solution (10 m m citrate acid, pH 6.0) for 20 min. The sections were cooled to room temperature for 30 min and rinsed with deionized water for 5 min. Possible intrinsic endogenous peroxidase activities were blocked with 2% hydrogen peroxide.
