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For the following sections, positions of the nodes and the BS will be as shown in Figure 17.
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To establish the percentage of new-generated PGCs and GCs double-labelled for the different markers, serial sections from standard, enriched and deprived mice were examined systematically through the OB (3 sections positioned respectively at antero, medial and posterior level).
GAD67 signal intensity was measured by densitometry using ImageJ software (NIH, Bethesda, MD) on 3 sections (positioned respectively at antero, medial and posterior level) per animal and corrected to the background signal.
To calculate the total number of TH- positive cells in the GL, we applied a random sampling method on 3 sections (positioned respectively at antero, medial and posterior level) per animal; density and total number of TH- positive cells were calculated as described above.
All neural cells from EM grid sections positioned in the MB cortex were included in the analysis.
Images were taken with a FEI Tecnai G from at least three separate sections positioned to capture the cytoplasm of random cells (excluding the nucleus).
In the few cases where noticeable colour fading had occurred, this was confined to sections positioned at the very edges of the slide/coverslip.
A fourth section positions the department chair in the context of VPE organizations.
Using the PET scanner's integrated laser positioning system, 3 5 transverse sectioning positions were identified and marked in each rat.
Then, the subsequent section positions the runway crossing application in the broader context of agent-based modeling.
It is shown that the agreement is good between the predictions of the r-p-p and e-p-p models on the cross-section positions with maximum rotation.
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