In total, the developing seeds of 77 plants were analysed of which 17 in duplicate (see section Plant material) to test the reproducibility of the analysis, giving a total of 94 samples.
The inoculated heads were first kept in the dark for 24 h then incubated in a 16 h light/8 h darkness photoperiod at 20°C with light intensities similar to the ones used for plant development (see section Plant growth conditions).
In a few cases, pollination mode was inferred from flower color, flower size and orientation, corolla tube length, and the time when flowers are open (see the criteria in the section Plant taxon sampling and sequence alignment).
The seeds and plant samples of L. iberica were obtained from the surroundings of Elazig subdivision in the Elazig province of Turkey and were identified in the section plant taxonomy of the Department of Biology, Firat University, Elazig, Turkey, where voucher sample of this plant was also deposited.
The RNAscope ISH method yields highly sensitive and specific mRNA expression localizations on conventional FFPE sections of plant tissues.
Using the optimized sprayer methods to apply DHB and CHCA, respectively, on serial sections of plant root nodule tissue provided complementary detection of endogenous metabolites.
A selection of the more widely favoured houseplants follows, under two sections: foliage plants, some of which also bear interesting flowers; and flowering plants, species kept primarily for their flowers.
The section on plant growth comes 500 pages later and refers to cell division, but to no other cellular functions.
In the introductory section the plant is presented and the state of the art is reported.
Average density of adult P. vulgatissima per 35 cm sections of plants in the spring was 0.347±0.092 in plantations, and 0.365±0.164 in natural stands.
The growth conditions are described in the Methods section under "plant growth".
