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All labelling experiments were thereafter performed three times with sections placed in Eppendorf tubes.
In brief, after rehydrated in water, the paraffin sections placed in citric buffer (pH 6.0) and treated in a microwave.
Haematoxylin and eosin staining was determined on sections placed in filtered 0.1% Harris' haematoxylin for 3 minutes and gently rinsed in cool running water to extract excessive stain.
The paraffin blocks were cut and 10 μm sections placed in eppendorf tube at the Pathology department of the VU University Medical Centre in Amsterdam, The Netherlands, by Dr Erik Thunnissen.
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A rectangular gate section placed in a flume was given freedom to vibrate in the vertical direction.
Every 10 sections, one section was placed in an Eppendorf tube, on dry ice.
The ileal tissue was gently washed with normal saline at room temperature, cut into 5 × 5 mm sections, and placed in Cryomatrix (Thermo Scientific, Pittsburgh, PA) medium for frozen sectioning.
Three 10-μm sections were then cut from each tumor block without micro- or macro-dissection, and the sections were placed in sterile Eppendorf tubes.
In the configuration investigated, blowing and outlet sections are placed in the front of the box.
Data collection started as soon as the appendix sections were placed in the appropriate solution.
These sections were placed in contact with an imaging plate BAS-MS20255, Fujifilm, Tokyo, Japan).
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