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Those studies indicated that transplanted tissue could help connect healthy sections of the spinal cord, he said.
These laminae were first described by Rexed in transverse sections of the spinal cord of the cat on the basis of neuronal size, shape, cytological features and density.
In adult male rats, phrenic motoneurons were labeled retrogradely by intrapleural injection of Alexa 488-conjugated cholera toxin B. In thin (10 μm) frozen sections of the spinal cord, fluorescently-labeled phrenic motoneurons were visualized for laser capture microdissection (LCM).
Three randomly selected sections of the spinal cord L1-L3 in each animal (n = 3) were analyzed.
Successive sections of the spinal cord tissues were sliced at a thickness of 30 µm by using a cryo-microtome and adhered onto gelatin-pretreated slides.
Coronal sections of the spinal cord taken from animals receiving either moderate or severe displacement injuries are shown in Figure 2b.
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A cross section of the spinal cord reveals white matter arranged around a butterfly-shaped area of gray matter.
Another subdural fluid collection was present in the anterior section of the spinal cord (at C5 D1 level) with a consequent posterior dislocation.
The dorsal horn (posterior), where primary nociceptive afferent nerve fibers project, contains laminae I to VI, whereas the ventral horn (anterior), comprising the motor neurons, contains laminae VII to IX. Lamina X surrounds the central canal (Figure 1). Figure 1 Rexed's laminae in a cross-section of the spinal cord at approximately the level of the seventh cervical vertebra (C7) with permission.
The inflammatory index was determined from the number and size of inflammatory lesions on an average of twenty complete cross-sections of the spinal cord and the brain of each animal, as previously described [16].
This small percentage of retracted nodes could be attributed to mechanical stress from sample handling during extraction and sectioning of the spinal tissues.
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