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During that time, light bleaches some sections of the leaf and alters the natural pigments in others.
Standard paraffin sections of the leaf blade were prepared and observed with a conventional optical microscope.
In paraffin sections of the leaf blade, four lacunae were observed in the midvein of the three Pubi-EXG1 plants out of three examined, whereas the control plant showed two lacunae (Figure 3e,f).
A. deserti loci were divided into 6 major clusters based on expression patterns across four PD sections of the leaf (Methods).
After herbivore feeding on the leaf base, (E)- α-bergamotene and (E)- β-farnesene (TPS10) and (E)- β-caryophyllene (TPS23), were produced by all sections of the leaf.
However, herbivory restricted to the middle of the leaf led to the emission of sesquiterpenes from only the middle and upper sections of the leaf; no sesquiterpenes were released from the leaf base.
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The factors were represented by season, the station and tissue type (adult sheaths and blades and intermediate leaves) and in the case of tannin cells, by the position of the section on the leaf.
For microscopy, cross-sections of the leaf mid rip were stained with 0.5% rose bengal dissolved in 5% aqueous ethanol for 15 sec and were washed with ddH2O (Saha et al. [1988]).
e, f A transverse section of the leaf blade with two lacunae in the midvein of the control plant (e) or the leaf blade with four lacunae in the midvein of the Pubi-EXG1 #18 plant (Taichung 65) (f).
The samples were placed in the embedding media such that a cross section of the leaf was obtained.
Water flow through any section of the leaf is driven by the difference in water potential between that section and the two bounding regions.
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