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Our study showed apoptosis of few photoreceptors, but the sections of the eye also revealed the absence of extensive apoptosis in the photoreceptor cell layer, despite the presence of diffuse photoreceptor mitochondrial oxidative stress (Fig. 4) [36].
This work is based on the finding that besides antero-posterior planes, coronal or near-coronal sections of the eye are attainable, resolving the pupil in real time, clearly and separately from the strongly reflective lens (Fig. 1, Video 1).
Serial 4-µm-thick sections of the eye were obtained and stained with hematoxylin and eosin.
The sections of the eye were incubated for 1 hour in a 5% Normal Donkey serum in 0.3% Triton X-100 in PBS, pH 7.4, followed by an overnight incubation with the mouse monoclonal antibody to amyloid beta which was made in 1% Normal Donkey Serum in 0.3% Triton X-100 in PBS.
Examination of differentiation by Elav staining confirmed that scrib- + RasACT + aPKCCAAXDN tumour tissue remained undifferentiated in the basal sections of the eye disc.
Table 2 summarizes the analysis of hematoxylin and eosin stained sections of the eye from E13.5 and E14.5 Sall2−/− null and Sall2+/− heterozygous control embryos, which revealed a penetrant bilateral phenotype in the homozygous Sall2−/− embryos.
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Imagine our surprise when upon completing histological sections of the eyes, we found that despite having a normal lens and eyeball, the fish lacked photoreceptors in their retinas.
Serial sections of the eyes were cut at 10 µm thickness on a cryostat (CM1850; Leica, Heidelberger, Nussloch, Germany) at −20 C, and prepared for staining.
This view is again consistent with an earlier report of Drosophila's switch-like torque responses between bilaterally oscillating bars [16], albeit such stimuli moved differently and covered smaller sections of the eyes than the flow fields used here.
For the pictures of the wings and the histological sections of the eyes, a Zeiss Axiophot Microscope was used.
(1) Paraffin sections of the eyes of three Sinocyclocheilus species: histological studies on eyes were performed from paraffin sections and hematoxylin and eosin (H&E) staining.
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item of the eye
sections of the ocular
sections of the visual
sections of the ride
sections of the show
sections of the skin
sections of the company
sections of the city
sections of the crowd
sections of the steel
sections of the government
sections of the wall
sections of the railroad
sections of the school
sections of the book
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