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A mixture of the oligonucleotides (10 ng each) was used in a first-round ten cycles of PCR procedure using pfu polymerase to generate most sections of the coding sequence.
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Using the data from MRSfinder, MRS locations were plotted on a section of sequence extending 1000 bp upstream of the translation start site (ATG codon) through the first 400 bp of the coding sequence (CDS) from each C. elegans gene.
The sequence of the coding sequence was verified (MWG Biotech).
Here, we describe a method for the synthesis of fluorescent fusion proteins using transposable elements, which can randomly integrate in the internal sections of the protein coding sequence to produce full-length fluorescent fusion proteins expressed at endogenous levels.
By means of RACE PCR, the coding sequence of these fragments was extended towards both the 5' and 3' ends of the coding sequences.
Nagase, T. et al. Prediction of the coding sequences of unidentified human genes.
The two alleles of the SNP rs6746030 alter the coding sequence of the sodium channel Nav1.7.
Sequencing of this region highlighted a synonymous single base change (T→C) within the coding sequence of exon 3 (see Figure 2), and a 160 bp section of sequence immediately upstream of exon 3 present in SHR but not WKY.
A graphical view of the mutations distribution along the coding sequence can be displayed in the 'statistics' section.
A cDNA clone containing the coding sequence of the rat cannabinoid receptor type 1 (CB1) (I.M.A.G.E. expressed sequence tag clone, GI Accession #11375084) was linearized after sequence verification.
To construct 35S TT2-GFP, the coding sequence of TT2 was cloned into pCAMBIA235S TT2-GFP
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