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In order to study the relationship between crypt loss and immune-cell infiltration, serial sections of slides used for automated crypt analysis were stained for neutrophils, macrophages, T cells and B cells.
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Some of Hatley's other choices however are unwieldy and distracting, notably the luridly tropical backdrop for the act II vision scene with a flaming sunset and giant purple daisies, and the sections of sliding set, which create a hyperactive confusion of perspective.
Their study, published in the Geological Society of America Bulletin, suggests that during a collapse, different sections of sliding debris can reach different velocities.
The area of the lateral ventricles (approximate bregma range: 1.10 0.38 mm) and aqueduct of Sylvius (approximate bregma range: –3.88 to –4.84 mm) was determined by tracing the outline of the area of interest in at least four adjacent sections of slide-mounted brain tissue using Neurolucida (MBF, Villiston, VT).
Sections were incubated overnight under light protected vacuum to allow an optimal fixation of brain sections on slides.
For immunohistochemistry, the paraffin-embedded breast lesions were first sectioned on slides of 4 μm thickness and placed on SuperFrostPlus glass (Menzel Glāser, Germany).
Five randomly selected sections of the slides, cross-sections of lung parenchyma, were captured, digitized and evaluated using Image Pro-Plus 5.1 software (Media Cybernetics, Inc., Silver Spring, MD, USA).
Serial sections of the slides showed geminin had a similar distribution, but with a lower frequency 40-500% of Ki67-positive cells; Figure 5A).
Thus, two neighboring serial sections of these slides were stained with anti-GFAP monoclonal antibody (Chemicon International, Temecula, CA) and anti-BCCIP antibody respectively using routine immunohistochemistry (IHC) protocols.
Through transparent sections of the slide's roof, you catch bleary glimpses of the surrounding Olympic-scape, before plunging into darkness and jolting around 12 hairpin bends at speeds of up to 15mph (which feels much faster, believe me).
Early in March, while bicycling the dead streets of the town on Long Island where I live, I came across a Dumpster with sections of a sliding glass door sticking out.
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