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It detected Aβ plaques in sections of brain tissue from an animal model of AD and displayed low uptake in the brain and high uptake in the liver in normal mice.
To treat epilepsy, Wilder Penfield, an early-20th-century neurosurgeon, would remove sections of brain tissue while his patients fully conscious but locally anaesthetized told him what they experienced as he administered small shocks to different areas of their brains.
We examined the deposition of C3 complement protein in mouse brain by means of immunohistochemistry in frozen sections of brain tissue obtained at 48 h post-ischemia.
To identify β-APP accumulation in axons, coronal sections of brain tissue including basal ganglia, frontal cortex, and corpus callosum, were immunohistochemically stained with the monoclonal antibody anti-β-amyloid precursor protein 695 (Clone LN27, Zymed, South San Francisco, CA).
Analysis of stained sections of brain tissue showed that Rcan2 was widely expressed, and was particularly prominent in hypothalamic nuclei such as the ventromedial (VMH), dorsomedial (DMH), and paraventricular (PVH) hypothalamic nuclei (Figure 5A 5D).
In sections of brain tissue, ZO-1 labeling was detected in short linear segments, that may correspond to endothelial junctions of blood vessels, but no corresponding PLEKHA7 labeling was detectable at these sites (arrowhead in Fig. 3C-C″).
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Two weeks later, the nude mice were euthanized, and frozen sections of brain tissues were analyzed for fluorescence under a fluorescence microscope.
When they initially compared aldehyde-fixed sections of brain tissues from wild type (WT) and mice knocked out (KO) for NR2A they saw a similar cytoplasmic labeling by immunofluorescence labeling of both tissues.
Briefly, the paraffin embedded sections of brain tissues from the WT mice and their Ppt1-KI littermates as well as those from Ppt1 −/− mice were placed on glass slides.
Intact PC12 cells expressing Q23 or Q74 growing on collagen IV-coated plates and the sections of brain tissues from wild-type mice and HD transgenic mice were fixed with a solution containing 2% (v/v) glutaraldehyde and 4% formaldehyde in 0.1 M sodium cacodylate, pH 7.4, for 15 min at room temperature.
Played by Vincent Lindon with somber reserve, he is shown working late into the night, chain-smoking as he studies cross-sections of brain tissue in search of clues.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com