As the first method, Avé et al. (1997) succeeded in detecting lacZ activity in a paraffinized section of an embryo using low-melting point paraffin at 42°C.
The minor axis of the outermost chloroplast end of paddle-shaped cells is perpendicular to the circular line around the embryo and is oriented along a line that more or less forms a half circle in the bend region when a midsagittal cross-section of an embryo is observed.
(E ) Immunohistological analysis of γH2AX (red) and cleaved caspase 3 (CC3, green) on sagittal sections of an Nde1−/−Lis1+/− embryo and its wild-type littermate at E12.5.
A sagittal section of an E12.5 embryo (Fig. 4e) revealed Neph3 expression domains in the entire thalamus, the cerebellum anlage, and the pontal flexure.
TEM of transverse ultrathin sections of a resin-embedded embryo revealed general ultrastructure of the trunk including the position of the notochord (N), neural tube (NT) and muscle blocks (M) with respect to the main axial blood vessels (Fig. 1D).
The reduction of eya1 mRNA compromises the formation of the otocyst as shown in the confocal sections of a stage 32 embryo.
We defined a 300 pixel long and 15 pixel wide region from the approximate anterior end the midbrain to the approximate posterior end of the rostral hindbrain (both marked by dashed red lines in Figure 4A) on a darkfield picture taken from a sagittal sections of an E12.5 wild-type embryo hybridized with the radioactive mmu-miR-709 detection probe).
In our analysis, we used flattened embryos and captured on average 6 z-sections for each region of an embryo taken in 0.5 µm intervals, which effectively captured all the intron dots in each nucleus.
One can readily capture all organs in a set of 24 serial sections through such an embryo.
However, transgenic embryos were missing cartilage rings around the trachea and bronchi.. Transverse sections of transgenic embryo E18.5 tracheas showed collapsed airway lumen, and a shape and appearance similar to esophagus; however, the esophagus was still present.
