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We conclude that subjective leaf rank provides a relatively consistent, semiquantitative measure of areole size among other variables; that modal areole size is generally consistent across large sections of a leaf lamina; and that both approaches semiquantitative, subjective scoring; and fully quantitative, automated measurement have appropriate places in the study of leaf venation.
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(a) Microscopic detection of Bb in a cross-section of a leaf mid rip from a Bb-treated leaf at 200-fold magnification.
(B) The same leaf during the winter reddening process showing a dead attached insect (detail of the insect in C). (D) Cross-section of a leaf under optical microscope showing abaxial and adaxial hairs.
b The optical micrograph of transverse sections of a rice leaf, and green areas illustrate the distribution of chlorophyllous cells.
c The epifluorescence micrograph of transverse sections of a rice leaf, and chlorophyllous cells can be identified by the bright red fluorescence, scale bar =250 μm.
a The painting with a white rectangle indicating the area of a detail scan shown in d. b Detail of the painting, the white arrow indicates the location of the sample shown in c. c Light microscopic image (×500 magnification, dark field) of a cross-section taken from a shadowed part of a leaf.
Fig. 4 Transverse sections of leaf blade (a f) and leaf margin (g l).
To establish whether the larger leaf size in the PdGAUT12.1-KD lines was a result of increased cell number or cell expansion, we analyzed cross sections of leaf blades of the sixth leaf from the apex of the plant and hand cut cross sections of the 20th leaf.
Seven-micron-thick FFPE cross sections of maize leaf were cut using a Microm HM 315 microtome (Microm International, Walldorf, Germany) and applied to Fisherbrand Superfrost Plus slides (Thermo Fisher Scientific, Pittsburgh, Pennsylvania, USA).
Standard paraffin sections of the leaf blade were prepared and observed with a conventional optical microscope.
Optical sections of tobacco leaf epidermal cells or tobacco cell cultures were observed with a ×63 water immersion apochromatic objective (numerical aperture 1.2, Zeiss) fitted to an inverted confocal microscope (Axiovert 200 M, LSM510 META; Zeiss) at 25°C.
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