Exact(8)
As per Discovery protocol, the instrument used 5 μm thick deparaffinised tissue sections mounted on positive charged glass slides, with subsequent digestion with Protease I (Ventana Medical Systems, AZ. USA) digestion for 12 minutes.
Ultrathin sections mounted on one-hole grids were stained with lead citrate.
In each level, five sections of 5 μm thickness were collected, one for H&E staining, two sections mounted on polyethylene naphthalate (PEN) membrane slides and subsequently stained with Methyl Green for laser capture microdissection and two sections mounted on sialinated slides, left unstained and in paraffin-embedded state for subsequent histochemical examinations.
Paraffin embedded tissues were used for histological evaluation as described previously33,35,36,37, using 5 μm sections mounted on Superfrost Plus slides.
We report here a method by which histological sections mounted on glass slides can be imaged in the SEM at a resolution higher than that obtained using conventional light microscopy.
Ultrathin sections mounted on copper grids were stained with 1%% aqueous uranyl acetate (Ted Pella Inc.,) for 15 min and lead citrate (Electron Microscopy Sciences, USA) for 1.5 min.
Frozen tumor sections were sliced in 5-μm sections, mounted on glass, and incubated for 1 h with 111In(DOTA- βAla 2-JMV594 DOTA- βAla 2-JMV594 DOTA- βAla 2-JMV594
Lung tissues were then processed and embedded in paraffin, and five-micrometer sections mounted on slides.
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