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Representative paraffin-embedded sections from each case were selected for immunohistochemical analysis using monoclonal antibodies D2-40 AE1/AE3/AE1/AE3
Immunohistochemical studies were performed on representative paraffin sections from each case using microwave pre-treatment of slides for antigen retrieval.
Some series of sections from each case were processed for immunoperoxidase and others for immunofluorescence histochemistry.
Three separate sections from each case were examined by the immunohistochemistry method.
Method specificity of labelling of GPT neurons was affirmed by omission of primary antibodies in 'control' sections from each case.
Unstained sections from each case were also sent to another participating laboratory for blinded retesting by CISH ('outside CISH'outside
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For PCR, one 10 μm paraffin section from each case was dewaxed in xylene for 30 min at room temperature, rehydrated through graded ethanols (5 min each in 100%, 96%and70%0% ethanol) and air dried.
Enrichment of tumor cells was obtained by manual microdissection under light microscopy from one to ten sections for each case.
The length of the pial boundary of the pallium associated with neocortex was measured on each camera lucida drawing from the series of sections obtained from each case (Alvin Plan Measure; Figure 5, lines A-A′).
Figure 1 shows photomicrographs from an unlesioned rhesus monkey brain, on which the intended lesion extent is indicated by red coloring, and sections from each lesion case VL1 VL4 at corresponding stereotaxic levels.
Serial sections are taken from each case, 5 sections per block.
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