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As examined on sections, expression was very strong around the ventricular zone (vz, relatively thick), but much more diffuse in the mantle zone (mz), and showed some clear discontinuities separating vz progenitor domains (Fig. 1C, E, F, dotted lines on Fig. 1C).
It is important to note that in the aerial sections, expression of PtGS1.3 was clearly associated with samples enriched in vascular tissue, such as petioles (P1, P2 and P3) and stems (S1, S2 and S3) whereas lower levels of gene expression were observed in the leaf lamina in all sections examined.
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In the following sections, expressions for the probability of reaching the next waypoint and for Pr{HOa} are provided.
The list of microarray experiments where a given C. elegans gene is known to be differentially expressed can be extracted from the section "Expression Cluster" from the WormBase gene summary page for each gene.
In each section, expression, intensity of immunostaining and percentage of labeled cells were analyzed.
Hybridization and post-hybridization washes were performed as described in the previous section "expression experiments".
See details in supplementary data section "expression data analysis" and Additional file 1: Table S2 regarding data sets.
Expression data is exported from EMMA (cf. Section "Expression data") via an export script that can be initiated by a human curator within the EMMA web interface.
We have also added RT-qPCR primer sequences and a reference to how COOLAIR was measured to the Materials and methods section "Expression analysis".
In order to determine whether a gene had a transcript support, we used the results from the section "Expression analysis using EST and full-length cDNA".
Consistently with the main bud transcriptional profiles described in the previous section, expression analysis of these transcripts identified three major distinct clusters.
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