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No immunoreactive products were found on the sections (data not shown).
This loss was estimated to amount approximately 9%, as evaluated by quantifying cells on tissue sections (data not shown).
Colocalized expression of the SK2 and BK channels was confirmed in single optical sections (data not shown).
An increase in erythroid precursors was also evident in H&E-stained splenic sections (data not shown).
Immunohistochemical staining was significantly less in these sections (data not shown), although it was not completely absent because the antibody can bind to the truncated protein.
Expression of (CUG 60 RNA under the same conditions did not appreciably affect eye morphology in tissue sections (data not shown).
Moreover, the luciferase activity of MSCs gradually decreased and at 168 h after reperfusion, we could not find any LacZ-positive cells in the liver sections (data not shown).
In the in situ hybridization performed with adjacent sections, Fox1 expression was not detected in tissues where the EGFP signal was observed, whereas strong signal of Fox1 was detected mainly in neuronal tissues in the same sections (data not shown).
Identical results were obtained on mouse and monkey brain sections (data not shown) suggesting that the faint staining of class2 scFv on TG2-rich tissues (Figure 3F) is likely to relate to its poor affinity for TG2 of species other than human.
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The number of lesions in the ALT and MC groups did not significantly differ from the HC group (on average 3.4±0.3 lesions per cross section; data not shown).
However, we detected no staining whatsoever in stromal or epithelial cells from the few remnant normal mouse ducts away from the lesion but in the same section (data not shown).
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