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Serial coronal sections (40 µm) from the hypothalamus of colchicine-treated rats were divided into three groups, referred to as 'bins', each containing in a total of six SCN-inclusive sections at intervals 120 µm apart.
They were then microscopically evaluated in serial H&E stained sections at intervals of 150 μm [ 20].
Serial sections at intervals of 1 mm were sliced resulting in complete work-up of the entire lymph node.
Two sets of serial sections (at intervals of 120 μm) were mounted on slides (Superfrost PLUS), using a solution of 0.1% BSA (albumin bovine, minimum 98%) in PBS.
Images were collected as stacks of 16 optical sections at intervals of 0.5 μm using a DeltaVision Elite microscope (Applied Precision) with a 20× N.A. 0.75 air objective.
Five μm paraffin brain sections were cut in a coronal plane from the olfactory bulb to the beginning of the cerebellum, and sections at intervals of 1400 μm or intervals of 700 μm throughout the hippocampal region were selected.
Similar(54)
For quantitative analysis of these immunohistochemical staining, three sections at interval of 10 sections from each aorta (per mouse) were selected and at least five high-power fields were randomly captured per section.
Each tumor was sectioned at intervals of 0.5 to 0.7 cm, and all of the sections were routinely processed and embedded in paraffin for histological examination.
The sample was translated at a constant velocity of 1.2 μm/s in order to acquire a series of z-sections at intervals of approximately 0.65 μm.
All the lymph nodes were serially sectioned at intervals of 250 μm (mean four levels per block).
The remaining paraffin-embedded material of these nodes was completely step sectioned at intervals of 50 μm, stained by H&E and immunohistochemistry and revealed micrometastases in one case, whereas the other four nodes remained negative.
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