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Cross sections as described above were placed onto a double sided carbon sticky tab adhered to an Al stub.
In situ hybridization was performed on free-floating sections as described previously [14].
ICA was determined by immunofluorescent staining on human AB- positive pancreatic sections as described [36].
Immunohistochemistry was done on floating vibratome sections as described previously [20].
After washing, iRBC were overlaid on placental sections as described above.
BrdU-immunohistochemistry was performed on paraffin-embedded sections as described [27].
They were used for immunofluorescence and semi-thin sections, as described below.
On the next day, the brains were cut in serial coronal sections as described before.
The number of RGCs was determined quantitatively in representative retinal sections as described in the methods (Figure 1F).
Immunohistochemistry was performed on histological sections as described by Lapidot et al. [18] using alloreactive naïve and interacting colonies.
Interstitial fibrosis was quantified by digital morphometry on Masson Trichrome-stained paraffin-embedded sections as described [43].
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