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These lung sections are processed immediately after obtaining from thoracic surgeons (co-authors Drs. Rodney J. Landreneau, Mathew J. Schuchert) by the Tissue Core (co-author Dr. Joe Pilewski, director of Tissue Core).
Aortic sections are processed for analysis of endothelial nitric oxide synthase (e-NOS) expression using RT-PCR.
Similar(58)
Brain sections were processed for CRH mRNA in situ hybridization.
Serial striatal sections were processed for TH staining following the same procedure as above.
One-in-three series of these brain sections were processed for immunostaining.
To control for the specificity of immunostaining, some sections were processed as above but without the primary or secondary antibody.
Sections were processed as previously described [23].
Sections were processed for immunohistochemistry as follows.
Cortex and striatal sections were processed as described [58].
Briefly, sections were processed in parallel using identical antibody concentrations.
All sections were processed under the same standardized conditions.
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