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In progressing from the field delineation phase, where sections are held by single well, to the field development phase, where multiple new wells are added to a previously produced section, many of the pre-existing "parent" wells suffer significant reduction in productivity as a result of interference from fracturing operations in the neighboring "infill" wells.
Its two expansive sections are held from below by trusses.
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During this action, one of the 2/9th's sections was held up by fire from three Japanese machine gun positions.
Eight IA sessions were held over Monday and Tuesday, and eight SPSA sections were held over Wednesday and Thursday.
Antigen retrieval was performed using citrate buffer (pH 6.0) and sections were held in Tris-buffered saline (TBS).
Antigen retrieval was performed using citrate buffer (pH 6.0), and sections were held in Tris-buffered saline.
The year there were the most double sections was 1988, when two concurrent sessions each of the Bacteriology, Immunology, and Virology sections were held.
After microwave antigen retrieval in citrate buffer, pH 6.0, the sections were held in Tris buffer solution (TBS, 100 m M, pH 7.6).
In addition to the topical sections, there were several years where double sessions of certain sections were held in order to accommodate the number of abstracts for those sections.
To minimize the length of time tissue sections were held on the microdissection microscope stage, and cells were pressure catapulted into 30 μL RLT lysis buffer (RNeasy, Qiagen) in lots of 100.
Formalin-fixed sections were held at 90°C for 60 min. Each sample was transferred to a 15-ml polypropylene centrifuge tube and combined with a 20-fold excess of room-temperature dilution buffer (8 M urea, 250 mM NaCl, 10 mM sodium phosphate buffer, 1 mM imidazole) to dilute the SDS, β-ME, and Tris to concentrations compatible with Ni-NTA agarose bead binding.
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