Samples were then sectioned with a microtome (Leica CM1850, Leica Instruments, Nussloch, Germany) into slices of 30 μm thickness.
Samples were then dehydrated in acetone, embedded in Spurr's resin and sectioned with a diamond knife microtome (Reichert-Jung TM60).
CAD/CAM blocks of both zirconia ceramics were sectioned with a diamond saw (Buehler Ltd ,Lake Bluff, IL, USA) and sintered according to the manufacturer's instructions.
These samples were then buried in paraffin, and sectioned with a microtome (Leica 1515, Leica Instruments, Nussloch, Germany) into slices of 15 μm thickness.
All multi-rooted teeth were sectioned with a Lindemann burr (Komet Inc., Lemgo, Germany) under copious irrigation with sterile saline to minimize extraction trauma.
The PVC master membranes were sectioned with a cork borer (10 Mm diameter) and glued to a polyethylene tube (3°Cm length, 8 Mm I.D).
For immunofluorescent staining, kidneys were sectioned with a cryostat.
Samples were embedded in Spurr's resin [43] and sectioned with a diamond knife.
The sections were then cryoprotected in sucrose (10%, 20% and 30%), and sectioned with a cryostat.
The material was sectioned with a glass knife using a Leica UC6 ultramicrotome (Leica).
Samples were processed for EM, embedded in Epon, and sectioned with a Leica Ultracut S ultramicrotome.
