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Five visual fields of the dorsal raphe nucleus in each section were selected for examination (× 40).
At least five representative fields (×40 objective on an Olympus Vanox AHBT3 microscope, Aartselaar, Belgium) within each section were selected for assessment.
The 10 most vascular areas within a section were selected for evaluation of angiogenesis, and vessels labelled with the anti-CD34 antibody or the anti-CD105 antibody were counted under light microscopy with a 200-fold magnification.
Slides were scanned using an Aperio CS Scanscope (Aperio Technologies Inc., Vista, CA); 20 randomly selected fixed-size fields from each section were selected for quantification by overlaying the tissue with a numbered grid.
IMVD was determined with an anti-CD34 monoclonal antibody QBEnd10 (mouse IgG1, kappa; DAKO Japan) diluted at 1 50; the 10 most vascular areas within a section were selected for evaluation of angiogenesis, and the average counts of CD34-positive vessels were recorded as IMVD in each case.
Similar(55)
The sectioning was performed with random start, and every 125th section was selected for analysis.
The transverse section was selected for scanning.
From each animal, every 12th section was selected for immunofluorescence staining for BrdU, Neu-N and Prox-1.
The gut section was selected for this analysis as it exhibited fluorescence deep below the surface of the tissue.
For pancreata analysis, sections (5 10μm) were cut throughout the entire pancreas and every tenth section was selected for histological and immunohistochemical examination.
For each analysis, sections (5 10μm) were cut throughout the length of the pancreas and every tenth section was selected for histological and immunohistochemical examination.
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