Exact(4)
The treated cells (as described in "Caspase assays" section) were fixed with 4%% paraformaldehyde for 10 min in situ and then stained with Hoechst 33258 (5 μg/mL) for 3 min at room temperature in the dark.
In other experiments cells were treated or not with Emp (defined in Cell Culture section) were fixed and incubated with anti-HIF-2α (ep190b, Novus-Biologicals) antibody.
Small pieces of each section were fixed in 10% (v/v) neutral buffered formalin for 24 hours for light microscopy or preserved in RNAlater® solution (Ambion, Austin, USA) for RT-qPCR analysis.
The remaining tissue from the frozen section as well as the lymph nodes that did not undergo frozen section were fixed in 4% buffered formalin overnight, processed, and paraffin embedded; 24 serial sections per block were cut and stained with hematoxylin/eosin depending on the size of the lymph nodes.
Similar(56)
Each section was fixed in methanol and stained in Mayer's hematoxylin.
Additional sections were fixed with glutaraldehyde and post-fixed with OsO4.
Sections were fixed in 1 1 acetone-to-methanol and incubated with PNA FISH probes at 55 °C.
Tissue sections were fixed in 10% buffered formalin and embedded in paraffin.
Frozen sections were fixed in 10% formamide.
Sections were fixed in 4% paraformaldehyde.
Ileum sections were fixed with 4% paraformaldehyde.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com