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Optical density measurements of binding in 15 brain regions on each section were evaluated by 1- and 2-way analysis of variance.
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The accuracy of each option in predicting the distribution of stress/strain in the cross section was evaluated by comparing the variation of the axial force in a critical beam following the removal of two columns.
Significance of the enrichment of these targets in the clusters obtained by the SOTA server (see previous section) was evaluated by means of a binomial distribution test.
A grid consisting of 20 70 points (depending on size of the tissue sample) were superimposed on each tissue section using a projection microscope and the entire section was evaluated by moving the grid in a random, systematic way.
Each section was evaluated by two blinded observers and then correlated.
For error control, the pathological section was evaluated by two observers separately; if any disagreement occurred, the result was judged by the third pathologist.
Each section was evaluated by three different blinded assessors (H.J., S.C., G.S). and discrepancies in the score assignment were solved by consensus in each case.
The staining intensity in each section was evaluated by three pathologists and graded according to the average percentage of positive cells in three randomly selected fields: – (0 5% cells stained), + (5%-20% cells stained), ++ (20%-50 20%-50 stained) and +++ (>50% cells stained).
After macroscopic inspection of the trachea, sections were evaluated by light microscopy.
Paraffin-embedded sections were evaluated by immunohistochemistry. Pathological findings were prospectively evaluated following the Japanese classification of biliary tract cancers (Miyazaki et al, 2015).
These selected sections were evaluated by two independent graders in the RIRRC who were informed of the patients' diagnosis but masked to the visits that were graded.
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