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The reliability of the UI symptom severity section and attitude section were assessed using Cronbach's alpha and was found to be 0.73 and 0.82, respectively.
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Each section is assessed using multiple choice questions with 4 or 5 optional responses, of which only one is correct.
The sections were assessed using light microscopy in a blinded manner by at least two of the authors.
The sections were assessed using a light microscope in a blinded fashion by at least two of the authors.
The sections were assessed using a Nikon Eclipse 80i microscope at ×200 view and a Nikon Digital Sight DS-U1 camera (Nikon UK, Kingston on Thames, UK).
Fluorochrome labelled bone sections were assessed using a triple filter (Zeiss filter set no. 25, Zeiss Axioplan, Carl Zeiss AG) and a fluorescent lamp.
Comparisons of nonparametric data from the modified Mankin histological scoring of the stained tissue sections were assessed using the Mann–Whitney U-test.
Haematoxylin and eosin-stained sections were assessed using the modified Sydney system for evaluating gastric changes related to Helicobacter pylori [ 42].
The presence of apoptotic cells in the lung sections were assessed using a TUNEL staining kit (Roche Diagnostics) according to the manufacturer's instructions.
The total area of the sheath section evaluated and the number of positive labelled nuclei on each of these three sections were assessed using a KS400, version 3.0, image-analysis system within the area of the screen at × 20 magnification.
Sections were assessed using a Nikon Eclipse E600 microscope equipped with a digital camera.> > -wrap-foot> aEmployed in IF immunofluorescence or IH immunohistochemistry bProvided by L. Sorokin cKindly provided by K. von der Mark (Erlangen) Immunofluorescence staining was performed on non-perfused, snap frozen tissues.
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