This classification was performed according to predictions in KEGG for the distribution of reactions in the collection of species (as described earlier in the section 'Retrieving a set of the human metabolic reactions').
My only serious comment would be that the authors refer to species' taxonomy as if it perfectly reflects species' phylogeny (e.g. the end of the first paragraph in the introduction and the first paragraph in the section "retrieve higher taxonomic names").
Immunostaining of Pis1 in the tissue sections retrieved with citrate buffer at both low and high temperatures was faint, while the sections retrieved with Tris-EDTA buffer gave stronger Pis1 immunoreactivity and a desirable light background at high temperature.
After water washing, sections retrieved the antigen with citrate buffer pH 6.0 in microwave for thirteen minutes.
CD68 (KP1, DAKO, Denmark), CD14 (M0825 clone TUK4, DAKO), IL-1β (Abcam ab8320), IL-6 (Abcam ab9324) and TNF (PeproTech 500-M26) staining was performed on sections retrieved in PT101 Link Pre-Treatment Module (Dako), as previously described [ 37].
More in depth, Bamboo and Georoy, the two protocols considered that will be described in the following sections, retrieve resources according to a distributed mechanism where, to speed up the lookup process and make it suitable to an unreliable scenario like the one addressed by our study, a replication methodology for managing multiple copies of the same resource has been introduced.
RESPONSE: Using the keyword search (described in "Extraction of miRNA-gene relations for Alzheimer's diseases from full MEDLINE" section) we retrieved 124 abstracts.
Minor questions RESPONSE: Using the keyword search (described in "Extraction of miRNA-gene relations for Alzheimer's diseases from full MEDLINE" section) we retrieved 124 abstracts.
It is composed of three sections, i.e., the searching section, the interaction retrieving section and the network analysis section.
After 2 weeks, the experimental sections were retrieved, sliced into thin sections 3.5 μm in average thickness, decalcified, and stained with hematoxylin eosin.
