He looked up homosexuality in the family dictionary, then took to going to libraries and planting himself in the sexuality section of the stacks.
Puncta with any portion appearing outside the boundaries of the terminal were removed from the analysis sample, as were those falling within the first and last optical sections of the stack as deconvolution is not reliable at these positions.
SEM-EDX analyses were performed on the fractured cross section of the YSZ/NiO YSZ/NiO stack.
Three wallaby samples and six dunnart samples contained a total of twelve co-localized signals in a single section, but none of them was visible in the previous or following section of the image stack (Figure 5A 5F).
The first approach involves performing 2-D image processing operations on the individual optical sections of the image stack.
The positions of these labels were then compared against the green Alexa 488 rod opsin label in all eight sections of the digital stack.
The near-surface faults and the larger fault that was continued to larger times were also imaged better in the migrated section of the FO-CDS stacked result.
For 3D reconstructions of serial sections, the stack of such segmented images was aligned with the SIFT linear elastic alignment algorithm (Fiji, [40]) and was reconstructed in Imaris 6.3.1 (Bitplane), using smoothing surface area detail level equal to 2 nm.
For quantitative analysis of the colocalization data each optical section of a stack was analysed with the "Coloc" module of the Imaris image analysis software (IMARIS 7.0.0, Bitplane, Zurich, Switzerland).
Meso-endo transitional arrangements, as seen in longitudinal section of the polyp, have elliptical or lachrymiform lacunae organized in a stack (reminiscent of a section through the stacks of cisternae) distally and transition, through a distinct constriction and sigmate-curve, to mesogleal pleats proximally (Fig. 1i j; Morphbank collection 851276; dimensions in Table 1).
