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If this all sounds good to you, you can nab the V CAST download right from the VZ Today section of the browser.
In brief the indexed file is put on a website accessible via HTTP, HTTPS or FTP, and a line describing the file type and data location in the form: track type=bigBed bigDataUrl= http://srvr/myData.bb is entered in the custom track section of the browser.
The Favorites menu is found in the Favorites/Bookmarks section of the browser (upper-right corner).
Access the Tools menu of IE. Launch Internet Explorer (IE) and select "Tools" from the menu bar at the upper lefthand section of the browser window.
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Restart the web server with: sudo service apache2 restart sudo service apache2 restart You will now be able to see the alignment track in the 'Select Tracks' section of the genome browser page (remember that only chromosome 1 is represented in the downloaded file!).
Refined searches were then performed using the rodent section of the NCBI taxonomic browser and BLAST [ 133] searches on euarchontan assembled genomes (mouse, rat, rabbit, human and rhesus macaque).
For protein or RNA coding genes, functional annotations are provided in the 'Region' and 'Details' sections of the main browser window.
(The "help" section of your browser will tell you how to make the cookies go away).
If you cannot find the pop-up blocker settings, check the support or troubleshooting section of your browser manufacturer's website.
If you do not drag it slowly, your browser may not scroll up to the top right section of your browser page.
In order to retrieve RefSeq sequences using Entrez query, we used nucleotide section of NCBI browser (http://www.ncbi.nlm.nih.gov/nuccore) with a command (srcdb_refseq_reviewed [prop] & mRNA).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com