Finally, although often cited in the methodological section of case studies, books are not considered which concentrate on data analysis in qualitative research per se (Miles et al. 2014; Corbin and Strauss 2015).
Furthermore, we created our own section of case based education.
Several sections of cases 2, 4 and 6 (Table 1) did not show a proper neuronal immunostaining, so these brains were discarded for the stereological study.
However, most of the other case characteristics were similar, regardless of the mode of ascertainment, suggesting that either method will capture a similar cross-section of cases.
The immunohistochemical study of 11 frozen sections of cases revealed unequivocal positivity in 10 cases.
The assessment of similar regions in the same sections of cases examined with pTDP43 and iTDP43 antibodies at high resolution is shown in Figure 4.
A joint assessment of IHC labeled sections of cases ranging from Braak stage 0 to VI was carried out around a multi-headed microscope.
Immunofluorescent dual labelling was carried out on selected, representative frontal lobe sections of cases with 4OPRI, 6OPRI, P102L, A117V, D178N, E200K mutations and sCJD 129MM, MV, and VV.
Direct comparisons of similar regions in the same sections of cases examined with pTDP43 and iTDP43 demonstrated more pathological inclusions with pTDP43, as well as a clearer distribution of these in the cortical sections.
As shown in Figure 2 (panel B), in serial sections of cases with high SERPINB3 mRNA, a strong reactivity for cytoplasmic SERPINB3, TGF- β1 and also for β-catenin was observed by immunohistochemistry.
