Only a few years after the amendment's ratification, this section was put into effect.
The crowd cheered each time a new section was put in place.
A modification of this approach involves "multiple shooting," where several Poincaré sections are put in phase space, and one successively integrates from one to the next and concatenates the results [59, 75].
Explants from roots and leaves (approximately 1 cm long) were excised in sterile conditions and sections were put in 60 mm Petri dishes with callus-induction medium (MS basal medium supplemented with 1 mg L−1 2,4- dichlorophenoxyacetic acid (2,4-D), 3% sucrose, 0.8% agar, pH 5.8) at 24°C with a 16∶8 h photoperiod.
First, two light blue h 61 * w 41 cm Styrofoam sections were put into place above the lower sheet flush with the sides of the tunnel, creating a h 61 * w 41 cm centred opening in the upper-half of the obstacle (Figure 1, trial 1).
Sections were put on glow-discharged carbon-coated formvar grids and imaged with a Tecnai G2 Sphera (FEI) electron microscope.
Five-micrometer-thick sections were put on poly-L-lysine-coated slides and stained with hematoxylin and eosin.
Ribbons of serial sections were put centered on 2 × 1 mm copper palladium slot grids (Agar Scientific Ltd ,UK).
The thick sections were put in Karnovsky's fixative (1% paraformaldehyde/3% glutaraldehyde/0.1 M sodium cacodylate buffer, pH 7.4) at 4°C overnight.
For dehydration prior to LCM, sections are put through a series of solvents also contained in nuclease-free 5-slide plastic mailers.
After treatment with 3% hydrogen peroxide for 30 min, the sections were put in a high-pressure environment for antigen retrieval.
