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Each root was cut in 10 mm section from the root tip, weighed 0.3 g, and was homogenized in liquid nitrogen.
At first, the sample was washed twice with distilled water, cut apical (±10 mm section) from the root tip, weighed, and soaked in 1.5 mL of 2 mM HNO3 solution for 24 h.
(A ) Transverse section from the root meristem of the PWER::GUS transgenic plant.
Tissue 3 mm from the root tip which contains meristematic cells and a further 1 cm section from the root containing non-meristematic cells were collected.
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The crowns were sectioned from the roots with a diamond bur at the buccal cementoenamel junction, and each crown was cut longitudinally in an occlusogingival direction to obtain two buccal enamel surfaces.
Sections from the roots, stems, leaves, petioles, flowers, and fruits of the plants were taken; for the non-stem or -root portions of the plants, the parts closest to the plant roots were selected.
Twist each section starting from the roots and working all the way to the tips.
Sections from the main root and intact lateral roots of four-week-old B. juncea plants expressing mRFP1 in response to Ni2+ were analyzed by epifluorescence and light microscope.
The least squares (LS) approximation of the upper part of the figure is based on data from section B. The root mean square error for X is RMSE x = 248 cm and for Y is RMSE y = 38 cm.
(A) Radicle transverse section, ∼1.5 cm from the root tip.
(C) Large lateral root transverse section ∼2 cm from the root tip.
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