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An expert panel of five physical therapists modified the section for clarity and natural language of physical therapists and reviewed the section for validation of content validity for physical therapists.
This layer is responsible for: (1) checkpointing tasks to account for task failure and allow restarts in remote nodes, without any source code modification, through the use of VMs; (2) providing different options for partitioning input data, chosen by the user; (3) local sampling at the server (more details afterwards in this section), for validation purposes.
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Having segmented each nucleus, we then use surface curvature, in section 3.6, for validation.
Classification as strong evidence requires additional, independent evidence, that the identified sites function in vivo (see section for cross-validation).
Twenty genes from biologically interesting pathways (discussed further in the next section) were chosen for validation of microarray data by qRT-PCR; of these 20 genes, 10 were downregulated (log2fold change range -0.27 to −5.50) and 8 upregulated (log2fold change range 0.40 to 1.26) in RS cells, while 2 showed no statistically significant change in expression between the two cell types (Table 1).
After completion of experiments, fresh-frozen brains were cryostat-cut into coronal 20 µm sections for further validation of the extent of lesion using Cresyl violet staining, as previously described [9], [10].
Fresh-frozen brains were cryostat-cut into 20 µm coronal sections for further validation of the location of recording track into the GP, STN and SNr as previously described [15], [18].
(PDF 506 kb) Additional file 17: Primers of all the genes described in the result section for qRT-PCR validation.
The respective CIDI section E was used for validation purposes.
> Using the approach described in the section 'Selection of InDels for validation and primer design' of Materials and methods, 3029 candidate InDels were selected and primers were designed for PCR validation (Supplementary Table S1).
Section 2.3 describes our method for cone segmentation, and Section 2.4 outlines the method for validation.
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