No swelling of the nucleus is due to permeabilization of the NPC to naturally impermeable macromolecules, e.g., PVP and large nuclear proteins (see the next section for experimental confirmation), which results in an isotonic condition across the NE.
Invasive cervical cancer biopsies were sectioned using a 'sandwich' approach, whereby inner tumour sections were destined for HPV testing and outer sections for histological confirmation of tumour tissue.
Biopsies were sectioned using a 'sandwich' approach, whereby inner tumour sections were destined for HPV testing and outer sections for histological confirmation of tumour tissue (see above).
Counterstaining of cell nuclei in tissue sections for morphological confirmation was performed with the haematoxylin solution for 1 min and then rinsed with distilled water.
The remaining half of the samples were fixed, embedded, and sectioned for histological confirmation of infection and to correlate with microCT scans.
When questions arouse concerning tissue morphology, H&E-stained sections were reviewed for confirmation.
Also, representative pieces of tumor tissue were fixed in formalin, embedded in paraffin, sectioned, and stained with H&E for confirmation of diagnosis.
In addition to tissue taken for culture, if donor or patient consent and/or ethical review permit (see Section 2.1), it is recommended that additional material is stored for: Confirmation of origin (authentication) (see Section 1.2.2).
Hematoxylin and eosin stained formalin-fixed paraffin-embedded sections were reviewed by a neuropathologist for confirmation of a diagnosis of high-grade glioma (glioblastoma WHO grade IV) as per World Health Organization criteria [ 18].
Sections for LMD were unfixed and unstained while adjacent sections were fixed and stained with haematoxylin for confirmation of morphology by a histopathologist.
In the next section, we will discuss the HD framework for confirmation and also compare it with Hempelian confirmation.
