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Frozen section and permanent diagnoses of the bile duct margin in gallbladder and bile duct carcinoma may be inconsistent in 25% of patients due to overdiagnosis of frozen section or new recognition of cancer cells by permanent histology.
Thin slices of tumour, taken between contiguous thin slices used for cryostat section and permanent sections (paraffin-embedded sections), were weighed, diced into 2 3 mm pieces, and washed three times with RPMI 1640 medium.
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Exclusively, the 200 patients whose SLNs were negative in frozen sections and permanent H&E-stained sections and in IHC staining had SLNB alone without further ALND.
Hypothesis.Frozen section diagnosis and permanent diagnosis of bile duct margin predict local recurrence after surgical resection of gallbladder or bile duct carcinoma.
Samples for frozen-section analysis and permanent histological analysis were obtained from the pseudocapsule, the membrane around the prosthesis, or tissue that was suspected of being infected.
Frozen sections were used to establish an rapid diagnosis intraoperatively, and permanent sections were used for diagnosis postoperatively.
Diagnosis of frozen and permanent section of bile duct margin, and local recurrence.
Despite extensive sampling on frozen and permanent sections, no evidence of invasion was identified.
We calculated the sensitivity, specificity, likelihood ratio of positive test results, and likelihood ratio of negative test results for serum CRP value, real-time PCR, and histopathology of frozen and permanent sections using the culture result as a reference.
We evaluated the properties of preoperative serum C-reactive protein (CRP), real-time polymerase chain reaction (PCR), and histopathological evaluation of frozen and permanent sections in clinical cases with culture-positive PJI.
"Underdiagnosis" was defined by Song et al. as those cases diagnosed as benign or borderline on frozen section and subsequently corrected at permanent pathology as borderline or malignant, respectively.
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