Sentence examples for seconds with final from inspiring English sources

The DNA was denatured at 95°C for 3 minutes, followed by 35 cycles at 96°C for 15 seconds, 60°C for 30 seconds, and 72°C for 30 seconds, with final extension at 72°C for 5 minutes.

Cycling parameters were, 50°C for 2 minutes, 95°C for 15 minutes, 40 cycles of 95°C for 10 seconds, followed by 55°C for 30 seconds and 72°C for 10 seconds with final melting at 95°C for 20 seconds.

Pentaplex PCR was performed with an initial 15-minute denaturation at 94°C, followed by 35 cycles at 94°C for 30 seconds, 55°C for 30 seconds, and 72°C for 30 seconds with final extension at 72°C for 10 minutes.

Assays were conducted using the PyroMark PCR Kit (Qiagen); conditions were: 95°C, 15 minutes; followed by 45 cycles of 94°C for 30 seconds, 56°C for 30 seconds and 72°C for 30 seconds; with final elongation at 72°C, 10 minutes.

These were carried out by a single reviewer, checked by a second with final consensus reached through a face-to-face discussion among the entire group.

PCR was performed with the following cycling protocol: 95°C for 5 seconds, followed by 20 cycles of 95°C for 30 seconds, 57°C for 30 seconds, and 72°C for 90 seconds, with a final elongation step at 72°C for 8 minutes.

Temperature cycling consisted of 98°C for 30 seconds followed by 12 cycles of 98°C for 30 seconds, 65°C for 30 seconds, 72°C for 30 seconds with a final Taq extension step at 72°C for 5 minutes.

Amplification was as follows: 98ºC for 30 seconds, followed by 15 cycles of: 98ºC 10 seconds, 60ºC for 30 seconds, and 72ºC for 15 seconds, with a final step of 10 minutes at 72ºC.

PCR conditions were: 94°C for 4 minutes, followed by 30 cycles of 94°C for 45 seconds, 60°C for 45 seconds and 72°C for 90 seconds, with a final extension at 72°C for 15 minutes.

PCR consisted of an initial denaturation step of 15 minutes at 95°C, followed by 35 cycles of 95°C for 30 seconds, 58°C for 30 seconds, and 72°C for 30 seconds, with a final extension step of 5 minutes at 72°C.

The amplification conditions included denaturation at 94°C for one minute and 33 cycles at 94°C for 30 seconds, 50°C for 30 seconds and 72°C for 25 seconds, with a final elongation at 72°C for seven minutes.