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Single FCS runs with 10 to 30 seconds were performed and average values were calculated.
Following a 1 minute, 95°C enzyme activation step 40 cycles of 95°C, 10 seconds and 60°C, 45 seconds were performed and a melting curve analysis followed.
After 5 minutes at 95°C for Taq DNA polymerase activation, a total of 45 cycles consisting of denaturation at 95°C for 15 seconds and annealing at 60°C for 30 seconds were performed.
Two PRF cycles of 180 seconds were performed after localization.
Again three trials of 40 seconds were performed.
Five measurements, each for 30 seconds, were performed for the individual samples and the values were averaged.
Similar(50)
An orbital agitation of three seconds was performed prior to each read.
Maximum number of knee bendings in 30 seconds was performed as described [ 28].
One sham recording (first) and one drugged recording (second) were performed on each recording day for each rat.
Among the later procedures, approximately two-thirds were performed as part of research projects.
The first was performed on April 23, the second on May 21.
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