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Each increment was light cured for 40 seconds using the XL 2500 curing unit (ESPE, Germany Norristow, PAA, USA).

Real-time bright-field microscopy and quantitative PMT detection indicated that that gram positive, gram negative and archaeal cells were effectively lyzed in a few seconds using the microfluidic chip protocol developed.

Ward also says that the key difference between near-time and real-time lies not only in the time lag seen with these services — after all, you can still get updates within a few seconds using the current methods — but in the way the current techniques interact with the server.

Anecdotal information indicates treatment duration per tree to be at least five minutes for skid hopping and ca. 15 seconds using the method described in this paper (P. Willemse, DOC, Twizel, 2011, pers. comm).. Aerial application also eliminates the dangers associated with manual control (P. Willemse, DOC, Twizel, 2011, pers. comm).

Following an initial denaturation step at 95°C for 15 minutes, 40 cycles were performed using 94°C for 20 seconds, annealing at 55°C for 20 seconds, extension at 68°C for 30 seconds, and fluorescence acquisition at 80°C for 10 seconds using the yellow channel (excitation at 530 nm, detection at 555 nm).

"The great advantage of this h index is the fact that you can get it in about 30 seconds" using the ISI Web of Knowledge, an online citation index, says Manuel Cardona (h = 86), a physicist at the Max Planck Institute for Solid State Research in Stuttgart, Germany.

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Third, using the 100-year time horizon keeps us from prioritizing non-CO2 climate pollution.

Many spill milk when first using the new jugs.

Add 1 1/2 cups lukewarm water and blend again, first using the fork and then your hands.

First, using the framework developed in Sect.

This year is the first using the new curriculum.

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