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First, is to note that the nominal delay used in the MoSP is lower h ˆ 0 nom = 9 seconds in comparison with the initial nominal model used in our approach h ˆ 0 nom = 23 seconds, this was necessary, because if we use a delay of h ˆ 0 nom = 23 seconds in the MoSP, this becomes unstable.
The following thermocycler touch-up PCR cycle was used: 95°C for 3 min, followed by 6 cycles of 95°C for 30 seconds, 50°C up to 55°C (0.3 increment/cycle) for 30 seconds, and 72°C for 30 seconds; this was followed by 34 cycles of 95°C for 30 seconds, 55°C for 30 seconds, 72°C for 30 seconds and a final extension at 72°C for 7 min.
The vial was vortexed for 10 seconds and rested for 10 seconds, this was repeated six times.
PCR conditions were: 95°C for 5 minutes, 10 cycles of 94°C for 15 seconds, 53°C for 30 seconds and 72°C for 45 seconds, this was repeated for another 25 cycles with the exception that the 72°C elongation step was increased by 5 seconds every cycle.
Although the blood sampling procedure was17 seconds shorter in KMC than incubator (153 vs 170 seconds), this was not significant.
If the patient was not able to perform the test within 12 seconds this was regarded as a reduced mobility and increased fall risk [ 26, 27].
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