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For immunoblot analysis, a constant OD of cells from each time point (5 OD units) were collected at the indicated time points, spun down immediately at 15000 g/30 seconds, the cell growth medium was removed and the cell pellet was frozen in liquid nitrogen.
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First, the cell sample may miss a pocket of abnormal cells or these cells may not get transferred to the slide.
At first, the cell was washed with distilled water.
Second, the cell viability was determined by MTT assays to evaluate the cellular toxicity of compounds.
First, the cell body was determined by segmentation.
First, the cell responses could become stronger at the new preferred orientation following the second adaptation.
First, the cell movement showed complex dynamics with anomalous diffusion where several characteristic timescales existed.
First, the cell membrane is internalized to produce endosomes.
Second, the cell surface GRP78 promotes the invasion of HCC in hepatocellular carcinoma cells.
First, the cells must be safe.
First, the cells are placed on a plastic dish coated with a gelatinous mix of collagen and other proteins.
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CEO of Professional Science Editing for Scientists @ prosciediting.com