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The tubes were first incubated for two minutes at 50°C (UNG reaction), then at 95°C for 15 minutes (UNG inactivation and polymerase activation) followed by 40 cycles consisting each of denaturation (94°C for 15 seconds), annealing (60°C for 30 seconds), extension (72°C for 30 seconds) and data acquisition (77°C for 15 seconds) steps.
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First words, first steps.
("First Steps").
These are good first steps".
His first steps were faltering.
These are useful first steps.
The first steps?
First Steps Outside Academia.
First Steps first?
Baby's first steps?
First steps.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com