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Thermal cycler conditions were 95°C for 30 seconds, followed by, 95°C for 10 seconds and 60°C for 30 seconds, repeated for 40 cycles.
Participants are instructed to stand as stationary as possible for 30 seconds, repeated for 8 trials, alternating eyes open and eyes closed.
Thermocycler conditions included an initial hold at 50°C for 2 minutes and then 95°C for 10 minutes, which was followed by a two-step PCR program of 95°C for 15 seconds and 60°C for 60 seconds repeated for 40 cycles on a Mx4000 system (Stratagene).
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An equal volume of acid-washed glass beads (size, 0.5 mm), vortexed for 30 seconds, then incubated on ice for 30 seconds, repeated this step for a total of 8 cycles.
Keep looking into your eyes, and then every 20 - 30 seconds repeat the question for about ten minutes.
Hold for 20 seconds, repeat up to 3 times for each leg. 1.
The melody of the first line was repeated for the second line of the stanza, a new melody being given to the next stanza; the music is syllabic.
So, if you can hold a particular stretch for 15 seconds, repeat it three more times.
Hold for 10 seconds, repeat opposite side.
Hold for 10 seconds, repeat 10 to 15 times.
Hold a comfortable stretch for 20 seconds, repeat it up to 3 times per each leg.
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